Size (kb): 13.6300001144409200
Vector: pPbac (phagemid)
Promoters: Promoter polyhedrin
Construct size (kb): 13.63000011444092
Features: marker(s): ampR
other: 5'-SmaI-Stuffer fragment-BamHI-3'
signal peptide coding region: human placental alkaline phosphatase
coding sequence: lacZ
vector permitting visual detection of recombinants
vector with sequences for protein export
Restriction digests of the clone give the following sizes (kb): BamHI--14.0; EcoRI--14.0; HindIII--8.0, 4.3, 1.0, 0.9.
The gene of interest can be inserted into pPbac by removal of the stuffer fragment which has a 5' SmaI site and a 3' BamHI site.
pPbac is a baculovirus transfer vector that facilitates secretion of recombinant proteins in insect cell systems based on the presence of the human placental alkaline phosphatase leader signal.
Mroczkowski BS, et al. Secretion of thermostable DNA polymerase using a novel baculovirus vector. J. Biol. Chem. 269: 13522-13528, 1994. PubMed: 8175786