pPbac (ATCC® 87314)

Applications: expression vectorvector permitting visual detection of recombinantsvector with sequences for protein export  /  Depositors: BS Mroczkowski

Permits and Restrictions

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Designations pPbac
Depositors BS Mroczkowski
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 13.6300001144409200
Vector: pPbac (phagemid)
Promoters: Promoter polyhedrin
Construction: pJVP10Z
Marker(s):ampR
Construct size (kb): 13.63000011444092
Features: marker(s): ampR
other: 5'-SmaI-Stuffer fragment-BamHI-3'
promoter: P10
promoter: polyhedrin
replicon: f1
signal peptide coding region: human placental alkaline phosphatase
coding sequence: lacZ
Applications
expression vector
vector permitting visual detection of recombinants
vector with sequences for protein export
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--14.0; EcoRI--14.0; HindIII--8.0, 4.3, 1.0, 0.9.
The gene of interest can be inserted into pPbac by removal of the stuffer fragment which has a 5' SmaI site and a 3' BamHI site.
pPbac is a baculovirus transfer vector that facilitates secretion of recombinant proteins in insect cell systems based on the presence of the human placental alkaline phosphatase leader signal.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mroczkowski BS, et al. Secretion of thermostable DNA polymerase using a novel baculovirus vector. J. Biol. Chem. 269: 13522-13528, 1994. PubMed: 8175786

Shipped freeze-dried