pSE2 (ATCC® 87313)

Applications: acentric fragmentation vector  /  Depositors: S Pestka

Permits and Restrictions

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Designations pSE2
Depositors S Pestka
Biosafety Level 1
Vector Information
Size (kb): 19.2500000000000000
Vector: pSE2 (plasmid)
Construction: pYAC4DRI+Sup11,SV2NEO; Alu repeat (Blur-13)_
Marker(s):G418R,URA3,ampR,HIS3
Construct size (kb): 19.25
Features: marker(s): G418 (SV2neo)
marker(s): HIS3
marker(s): URA3
marker(s): ampR
other: Alu repeat sequence
other: telomere
replicon: ARS:TRP1
replicon: pMB1
restriction site: BamHI
restriction site: EcoRI
restriction site: NotI
restriction site: SacI
centromere: CEN4:sup11
Applications
acentric fragmentation vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--19.3; NotI--19.3; SacI--19.3.
A Ura- yeast strain containing the desired YAC can be transformed with the 8.25 kb EcoRI-BamHI fragment of the vector, followed by selection on uracil-deficient media to form deletion variants of original clone.
The NotI and SacI sites are unique and may be used to rescue the right end of the YAC.
G418 resistance can also be used to select for transformed mammalian cells.
pSE1 (ATCC 87312) and pSE2 (ATCC 87313) differ only in the orientation of the Alu repeat sequence.
Yeast artificial chromosome (YAC) fragmentation vector allowing generation of deletions from the right end of YAC clones.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Cook JR, et al. Yeast artificial chromosome fragmentation vectors that utilize URA3 selection. Genet. Anal. Tech. Appl. 10: 109-112, 1993. PubMed: 8204303

Shipped freeze-dried
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