pPD112 (ATCC® 87226)

Applications: expression vectorvector with low copy number  /  Depositors: E Bremer

Permits and Restrictions

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Designations pPD112
Depositors E Bremer
Biosafety Level 1
Vector Information
Size (kb): 3.5299999713897710
Vector: pPD112 (plasmid)
Promoters: Promoter T7 gene 10
Construction: pPD100, pTrc97C
Marker(s):cmlR
Construct size (kb): 3.529999971389771
Features: marker(s): cmlR
promoter: T7 gene 10
replicon: pSC101
MCS: NcoI...HindIII
ribosome-binding site: lacZ
Applications
expression vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.5; HindIII--3.5; NcoI--2.9, 0.65.
pPD110 (ATCC 87224), pPD111 (ATCC 87225) and pPD112 (ATCC 87226) allow expression of inserts lacking transcriptional and translational initiation signals. These three vectors differ only in the reading frame of the ATG start codon.
Deposited for research purposes only. Before any commercial use, the depositor must be contacted.
A low copy number plasmid using the T7 gene 10 promoter for selective, overexpression of cloned genes in E. coli.
Media Medium 2272: LB Medium (Miller) with chloramphenicol 25 mcg/ml
Growth Conditions
Temperature: 37.0°C
References

Dersch P, et al. Low-copy-number T7 vectors for selective gene expression and efficient protein overproduction in Escherichia coli. FEMS Microbiol. Lett. 123: 19-26, 1994. PubMed: 7988888

Erhard Bremer, personal communication

Shipped freeze-dried
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