pUK (ATCC® 87209)

Applications: expression vector  /  Depositors: B Huang

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Designations pUK
Depositors B Huang
Biosafety Level 1
Vector Information
Size (kb): 2.4300000667572020
Vector: pUK (plasmid)
Promoters: Promoter tac
Construction: pUC19, pKK223-3
Marker(s):ampR
Construct size (kb): 2.430000066757202
Features: marker(s): ampR
promoter: tac
replicon: pMB1
MCS: HindIII-EcoRI
ribosome-binding site: Shine-Dalgarno
transcription terminator: rrnB
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.4; BamHI--2.4; HindIII--2.4.
A small, high-copy-number vector suitable for both in vitro (using the coupled transcription/translation system of Zubay) and in vivo expression.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Huang B, et al. A small, high-copy-number vector suitable for both in vitro and in vivo gene expression. Gene 151: 143-145, 1994. PubMed: 7828863

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
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