pIIIEx316 tRNA/3'ST (ATCC® 87178)

Applications: YC-type (centromeric) shuttle vectorvector permitting RNA synthesis in vitrovector permitting expression of small structured RNAsvector permitting production of single-stranded DNAvector permitting visual detection of recombinantsvector with low copy number  /  Depositors: DR Engelke

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Designations pIIIEx316 tRNA/3'ST
Depositors DR Engelke
Biosafety Level 1
Vector Information
Size (kb): 6.5999999046325680
Vector: pIIIEx316 tRNA/3'ST (plasmid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS316
Marker(s):URA3,ampR
Construct size (kb): 6.599999904632568
Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: SUP4 tRNA intragenic promoter
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
restriction site: BamHI
restriction site: EcoRI
restriction site: SalI
terminator: 3' stem and poly(T)
terminator: RPR1
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
vector permitting RNA synthesis in vitro
vector permitting expression of small structured RNAs
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.6; BamHI--6.6; HindIII--6.6.
Cloned inserts can be transcribed from the SUP4 tRNA gene intragenic promoter to produce structured pre-tRNA leaders fused to RNA corresponding to inserted sequences.
Vector contains a strong stem sequence following the cloning site, which is predicted to form a G+C-rich 6 bp RNA stem, followed by a poly(T) terminator sequence and RPR1 terminator.
One of a series of shuttle vectors (ATCC 87167 - 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number.
Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS316.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Good PD, Engelke DR. Yeast expression vectors using RNA polymerase III promoters. Gene 151: 209-214, 1994. PubMed: 7828876

Shipped freeze-dried
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