pIIIEx424 tRNA (ATCC® 87175)

Applications: YE-type (episomal) shuttle vectorvector permitting RNA synthesis in vitrovector permitting expression of small structured RNAsvector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: DR Engelke

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Designations pIIIEx424 tRNA
Depositors DR Engelke
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 7.3000001907348630
Intact vector size: 7.300
Type of vector: plasmid
Cloning sites: EcoRI
Polylinker sites:
Construction: pRS424
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): TRP1, ->, 468-1142
replicon: f1, ->, 1241-1707
insert detection: lacZ', <-
promoter for in vitro transcription: T7, ->, 1867-1886
restriction site: SalI
terminator: RPR1, <-
restriction site: EcoRI
promoter: SUP4 tRNA intragenic promoter, <-
restriction site: BamHI
promoter for in vitro transcription: T3, <-
promoter: lac, <-
replicon: pMB1
marker(s): ampR, <-
replicon: 2 micron
Vector: pIIIEx424 tRNA (plasmid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS424
Construct size (kb): 7.300000190734863
Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: SUP4 tRNA intragenic promoter
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
replicon: f1
replicon: pMB1
restriction site: BamHI
restriction site: EcoRI
restriction site: SalI
terminator: RPR1
YE-type (episomal) shuttle vector
vector permitting RNA synthesis in vitro
vector permitting expression of small structured RNAs
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): EcoRI--7.4; BamHI--7.4; HindIII--5.2, 2.1.
Cloned inserts can be transcribed from the SUP4 tRNA gene intragenic promoter to produce structured pre-tRNA leaders fused to RNA corresponding to inserted sequences.
One of a series of shuttle vectors (ATCC 87167 - 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number.
Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS424.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Good PD, Engelke DR. Yeast expression vectors using RNA polymerase III promoters. Gene 151: 209-214, 1994. PubMed: 7828876

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Shipping Information Distributed: freeze-dried
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