pIIIEx314 tRNA (ATCC® 87173)

Applications: YC-type (centromeric) shuttle vectorvector permitting RNA synthesis in vitrovector permitting expression of small structured RNAsvector permitting production of single-stranded DNAvector permitting visual detection of recombinantsvector with low copy number  /  Depositors: DR Engelke

Permits and Restrictions

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Designations pIIIEx314 tRNA
Depositors DR Engelke
Biosafety Level 1
Vector Information
Size (kb): 6.5000000000000000
Vector: pIIIEx314 tRNA (plasmid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS314
Marker(s):TRP1,ampR
Construct size (kb): 6.5
Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: SUP4 tRNA intragenic promoter
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
restriction site: BamHI
restriction site: EcoRI
restriction site: SalI
terminator: RPR1
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
vector permitting RNA synthesis in vitro
vector permitting expression of small structured RNAs
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.55; BamHI--6.55; HindIII--4.7, 1.7.
Cloned inserts can be transcribed from the SUP4 tRNA gene intragenic promoter to produce structured pre-tRNA leaders fused to RNA corresponding to inserted sequences.
One of a series of shuttle vectors (ATCC 87167 - 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number.
Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS314.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Good PD, Engelke DR. Yeast expression vectors using RNA polymerase III promoters. Gene 151: 209-214, 1994. PubMed: 7828876

Shipped freeze-dried
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