Size (kb): 7.5000000000000000
Vector: pIIIEx423 tRNA (plasmid)
Promoters: Promoter for in vitro transcription T7
Construct size (kb): 7.5
Features: insert detection: lacZ'
promoter: SUP4 tRNA intragenic promoter
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
restriction site: BamHI
restriction site: EcoRI
restriction site: SalI
YE-type (episomal) shuttle vector
vector permitting RNA synthesis in vitro
vector permitting expression of small structured RNAs
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): EcoRI--7.5; BamHI--7.5; HindIII--5.1, 2.2, 0.2.
Cloned inserts can be transcribed from the SUP4 tRNA gene intragenic promoter to produce structured pre-tRNA leaders fused to RNA corresponding to inserted sequences.
One of a series of shuttle vectors (ATCC 87167
- 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number.
Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS423.
Good PD, Engelke DR. Yeast expression vectors using RNA polymerase III promoters. Gene 151: 209-214, 1994. PubMed: 7828876