pExSec1 (ATCC® 87164)

Applications: expression vectorvector permitting RNA synthesis in vitrovector permitting construction of fusion proteinsvector permitting production of single-stranded DNAvector with sequences for protein export  /  Depositors: C Bruenen-Nieweler

Permits and Restrictions

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Designations pExSec1
Depositors C Bruenen-Nieweler
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 3.3810000419616700
DESCRIPTION OF VECTOR:
Intact vector size: 3.381
Type of vector: phagemid
Cloning sites: EcoRI SacI Asp718 KpnI SmaI BamHI
Polylinker sites: EcoRI SacI Asp718 KpnI SmaI BamHI
Construction: pUK21, pET3b, pEZZ18
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7, ->, 82-98
ribosome-binding site: Shine-Dalgarno, ->, 148-153
restriction site: NdeI, 159-164
leader peptide: synthetic ZZ sequence of protein A, ->, 162-641
MCS: EcoRI...BamHI, ->, 642-670
transcription terminator: T7, ->, 673-795
other: M13 intergenic region, ->
marker(s): kanR, ->
replicon: M13
Vector: pExSec1 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pUK21, pET3b, pEZZ18
Marker(s):kanR
Construct size (kb): 3.38100004196167
Features: leader peptide: synthetic ZZ sequence of protein A
marker(s): kanR
other: M13 intergenic region
promoter for in vitro transcription: T7
replicon: M13
MCS: EcoRI...BamHI
restriction site: NdeI
ribosome-binding site: Shine-Dalgarno
transcription terminator: T7
Applications
expression vector
vector permitting RNA synthesis in vitro
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
vector with sequences for protein export
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.4; BglI/EcoRI--3.1, 0.3; EcoRI--3.4; BglII/EcoRV--3.2, 0.2.
Expression and secretion vector for E. coli.
Constructed using M13 replication and kanR selection mechanisms from pUK21, T7 regulatory sequences from a 271 bp BglII-EcoRV fragment of pET3b and leader peptide sequence and multiple cloning sites from pEZZ18.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Brunen-Nieweler C, et al. Expression of the pheromone 3-encoding gene of Euplotes octocarinatus using a novel bacterial secretion vector. Gene 150: 187-192, 1994. PubMed: 7959050

Shipped frozen
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87164