pExSec1 (ATCC® 87164)

Applications: expression vectorvector permitting RNA synthesis in vitrovector permitting construction of fusion proteinsvector permitting production of single-stranded DNAvector with sequences for protein export  /  Depositors: C Bruenen-Nieweler

Designations pExSec1
Depositors C Bruenen-Nieweler
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 3.3810000419616700
DESCRIPTION OF VECTOR:
Intact vector size: 3.381
Type of vector: phagemid
Cloning sites: EcoRI SacI Asp718 KpnI SmaI BamHI
Polylinker sites: EcoRI SacI Asp718 KpnI SmaI BamHI
Construction: pUK21, pET3b, pEZZ18
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7, ->, 82-98
ribosome-binding site: Shine-Dalgarno, ->, 148-153
restriction site: NdeI, 159-164
leader peptide: synthetic ZZ sequence of protein A, ->, 162-641
MCS: EcoRI...BamHI, ->, 642-670
transcription terminator: T7, ->, 673-795
other: M13 intergenic region, ->
marker(s): kanR, ->
replicon: M13
Vector: pExSec1 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pUK21, pET3b, pEZZ18
Marker(s):kanR
Construct size (kb): 3.38100004196167
Features: leader peptide: synthetic ZZ sequence of protein A
marker(s): kanR
other: M13 intergenic region
promoter for in vitro transcription: T7
replicon: M13
MCS: EcoRI...BamHI
restriction site: NdeI
ribosome-binding site: Shine-Dalgarno
transcription terminator: T7
Applications
expression vector
vector permitting RNA synthesis in vitro
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
vector with sequences for protein export
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.4; BglI/EcoRI--3.1, 0.3; EcoRI--3.4; BglII/EcoRV--3.2, 0.2.
Expression and secretion vector for E. coli.
Constructed using M13 replication and kanR selection mechanisms from pUK21, T7 regulatory sequences from a 271 bp BglII-EcoRV fragment of pET3b and leader peptide sequence and multiple cloning sites from pEZZ18.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Brunen-Nieweler C, et al. Expression of the pheromone 3-encoding gene of Euplotes octocarinatus using a novel bacterial secretion vector. Gene 150: 187-192, 1994. PubMed: 7959050

Shipped frozen
Shipping Information Distributed: freeze-dried