pKSM718 (ATCC® 87163)

Applications: expression vectorvector permitting RNA synthesis in vitrovector permitting construction of fusion proteinsvector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: K Ippen-Ihler, S Maneewannakul

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Designations pKSM718
Depositors K Ippen-Ihler, S Maneewannakul
Biosafety Level 1
Vector Information
Size (kb): 3.5840001106262210
Vector: pKSM718 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM710, pUC18
Marker(s):ampR
Construct size (kb): 3.584000110626221
Features: insert detection: lacZalpha
marker(s): ampR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
MCS: DsaI...EcoRI
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
Applications
expression vector
vector permitting RNA synthesis in vitro
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.6; EcoRI--3.6; BglI--3.6.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglI--3.29; EcoRI--0.39; NcoI--0.33; PvuI--0.51, 3.04; PvuII--0.48.
Differs from pKSM717 (ATCC 87162) only in the orientation of the ampicillin resistance gene.
Expression vector allowing generation of N-terminal deletions or modifications of foreign DNA cloned into the multiple cloning site.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823

Shipped freeze-dried
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