pBSL128 (ATCC® 87144)

Applications: contains easily purifiable cassette(s) for construction omega-nptII  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL128
Depositors MF Alexeyev
Biosafety Level 1
Vector Information
Size (kb): 5.0599999427795410
Vector: pBSL128 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Marker(s):ampR,kanR
Construct size (kb): 5.059999942779541
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: nptII (kanR)
Applications
contains easily purifiable cassette(s) for construction omega-nptII
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 2.0; EcoRI--2.9, 2.1; KpnI--2.9, 2.0; MluI--3.0, 2.0; SstI--3.0, 2.0.
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA.
Plasmid useful for vector construction. Contains a nptII (kanR) cassette flanked by omega elements and multiple cloning sites duplicated in inverted orientation.
SphI and PstI sites in the nptII gene were removed by site-directed mutagenesis.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
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