pBSL97 (ATCC® 87140)

Applications: contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance  /  Depositors: MF Alexeyev

Designations pBSL97
Depositors MF Alexeyev
Biosafety Level 1
Vector Information
Size (kb): 4.3000001907348630
Vector: pBSL97 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Marker(s):ampR,kanR
Construct size (kb): 4.300000190734863
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI SacI...KpnI
coding sequence: nptII (kanR)
Applications
contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.6, 1.3; EcoRI--2.75, 1.4; KpnI--2.5, 1.45; MluI--2.85, 1.25; SstI--2.85, 1.25.
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA.
SphI and PstI sites in the nptII gene were removed by site-directed mutagenesis.
Contains a nptII (kanR) cassette flanked by multiple cloning sites duplicated in inverted orientation.
pBSL97 (ATCC 87140) and pBSL99 (ATCC 87141) differ in the orientations of the multiple cloning sites.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet