pBend4 (ATCC® 87122)

Applications: vector containing primer sites useful for sequencingvector for studying DNA-protein interactions  /  Depositors: C Zwieb

Permits and Restrictions

View Permits

Designations pBend4
Depositors C Zwieb
Biosafety Level 1
Vector Information
Size (kb): 3.2019999027252200
Vector: pBend4 (plasmid)
Promoters: Promoter T7 (phi10)
Construction: pBluescriptSK-
Marker(s):ampR
Construct size (kb): 3.20199990272522
Features: marker(s): ampR
promoter: T3
promoter: T7 (phi10)
replicon: pMB1
MCS: HindIII...EcoRI
Applications
vector containing primer sites useful for sequencing
vector for studying DNA-protein interactions
Comments
Restriction digests of the clone give the following sizes (kb): BglI--1.55, 1.25, 0.3, 0.24; EcoRI/HindIII--2.9, 0.27; XbaI--3.2.
Recommendation for verification: EcoRI+HindIII--2.95, 0.25; BglI--1.9, 1.3; XbaI--3.2.
Target DNA binding sites can be cloned into the vector and an array of fragments can be generated (by restriction digestion with different enzymes) that are equal in length, but differ in the position of the DNA binding site along the fragment.
The reduced mobility of different protein-fragment complexes can then by analyzed by gel electrophoresis to determine the degree of bending induced by protein binding.
One of three vectors (ATCC 87121-87123) designed for studying the amount of bending induced at a DNA binding site due to DNA-protein interactions. The three vectors differ only in the available cloning sites.
Vector constructed from pBend3 (ATCC 87121) by addition of SalI and HpaI sites at the SalI site in the polylinker.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Kim J, et al. Bending of DNA by gene-regulatory proteins: construction and use of a DNA bending vector. Gene 85: 15-23, 1989. PubMed: 2533576

Zwieb C, Adhya SImproved plasmid vectors for the analysis of protein-induced DNA bendingIn: Zwieb C, Adhya SMethods in Molecular Biology, Vol 30: DNA-Protein interactions: Principles and ProtocolsTotowa, NJThe Humana Press, Inc.pp. 281-294, 1994

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet