pJC20 plasmid in E. coli (ATCC® 87113)

Applications: expression vectorvector for generating nested deletions of cloned inserts  /  Depositors: J Clos

Permits and Restrictions

View Permits

Designations pJC20 plasmid in E. coli
Depositors J Clos
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.3399999141693120
Vector: pJC20 plasmid in E. coli (plasmid)
Promoters: Promoter for expression T7 (phi10)
Construction: pJC10
Marker(s):ampR
Construct size (kb): 2.339999914169312
Features: marker(s): ampR
promoter for expression: T7 (phi10)
replicon: pMB1
MCS: NdeI...ApaI
ribosome-binding site: Shine-Dalgarno
terminator: T phi
Applications
expression vector
vector for generating nested deletions of cloned inserts
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.3; HindIII--2.3; XbaI--2.3.
High-copy-number expression vector (T7-based) that can be used for preparation of nested deletions.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Clos J, Brandau S. pJC20 and pJC40--two high-copy-number vectors for T7 RNA polymerase-dependent expression of recombinant genes in Escherichia coli. Protein Expr. Purif. 5: 133-137, 1994. PubMed: 8054844

Clos J, et al. Molecular cloning and expression of hexameric Drosophila heat shock factor subject to negative regulation. Cell 63: 1085-1097, 1990. PubMed: 2257625

Shipped freeze-dried
Shipping Information

 Freeze dried plasmid in E. coli

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87113