YCpIF17 (ATCC® 87092)

Applications: YX-type (expression) shuttle vectorencodes an epitope tag for protein isolation or monitoring  /  Depositors: RW Davis, PK Foreman

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Designations YCpIF17
Depositors RW Davis, PK Foreman
Biosafety Level 1
Host Escherichia coli HB101
Vector Information
Size (kb): 5.30
Vector: YCpIF17 (plasmid)
Promoters: Promoter for expression GAL1
Construction: YCpIF15
Marker(s): TRP1,ampR
Construct size (kb): 5.30


Features:
marker(s):
TRP1
marker(s): ampR
promoter for expression: GAL1
replicon: ARS1
replicon: pMB1
MCS: SacI...ClaI
centromere: CEN4
epitope tag: hemaglutinin (HA), influenza virus
Applications
YX-type (expression) shuttle vector
encodes an epitope tag for protein isolation or monitoring
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--3.5, 1.8; BamHI--5.3; EcoRI--5.3.
Yeast expression vector allowing production of a protein tagged at the N-terminal end with a hemaglutinin epitope.
Vectors YCpIF15, YCpIF16 and YCpIF17 (ATCC 87090-87092) differ only in the reading frame of the multiple cloning site.
The encoded nine aa of the influenza virus hemaglutinin epitope are recognized by the monoclonal antibody 12CA5, allowing detection and affinity purification of the recombinant protein.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907

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