YCpIF7 (ATCC® 87084)

Applications: YX-type (expression) shuttle vector  /  Depositors: RW Davis, PK Foreman

Permits and Restrictions

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Designations YCpIF7
Depositors RW Davis, PK Foreman
Biosafety Level 1
Vector Information
Size (kb): 6.4000000953674320
Vector: YCpIF7 (plasmid)
Promoters: Promoter for expression GAL1
Construction: pUN95
Marker(s):ampR,HIS3
Construct size (kb): 6.400000095367432
Features: marker(s): HIS3
marker(s): ampR
promoter for expression: GAL1
replicon: ARS1
replicon: pMB1
MCS: SalI...SacI
centromere: CEN4
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--6.6; BamHI--4.2, 2.4; EcoRI--6.6.
One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter.
Vectors YCpIF7, YCpIF8 and YCpIF9 (ATCC 87084-87086) differ only in the reading frame of the multiple cloning site.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907

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