pBact-myc (ATCC® 87060)

Applications: expression vectorshuttle vectorvector permitting construction of fusion proteins  /  Depositors: A Matus

Permits and Restrictions

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Designations pBact-myc
Depositors A Matus
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.6180000305175780
DESCRIPTION OF VECTOR:
Intact vector size: 5.618
Type of vector: plasmid
Cloning sites: EcoRI ClaI
Polylinker sites: HindIII NcoI EcoRI ClaI HindIII
Construction: pBact16, pUC18, c-myc
Host range: Escherichia coli; mammalian cells
Features (with orientation and position when available):
promoter: beta-actin, ->
restriction site: HindIII
other: Kozak consensus sequence
restriction site: NcoI
other: c-myc epitope sequence, ->
restriction site: EcoRI
restriction site: ClaI
restriction site: HindIII
terminator: SV40 polyadenylation
replicon: pMB1
marker(s): ampR, <-
Vector: pBact-myc (plasmid)
Promoters: Promoter beta-actin
Construction: pBact16, pUC18, c-myc
Marker(s):ampR
Construct size (kb): 5.618000030517578
Features: marker(s): ampR
other: Kozak consensus sequence
other: c-myc epitope sequence
promoter: beta-actin
replicon: pMB1
restriction site: ClaI
restriction site: EcoRI
restriction site: HindIII
restriction site: NcoI
terminator: SV40 polyadenylation
Applications
expression vector
shuttle vector
vector permitting construction of fusion proteins
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--5.6, PstI--4.8, 0.45, 0.35; EcoRI--5.6.
Shuttle expression vector allowing production of a fusion protein containing an amino terminal c-myc epitope tag (FQKLISEEDLN), that can be used for immunological detection of the recombinant protein in transfected cells.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Cravchik A, Matus A. A novel strategy for the immunological tagging of cDNA constructs. Gene 137: 139-143, 1993. PubMed: 7506688

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Shipping Information Distributed: freeze-dried