pBact-myc (ATCC® 87060)

Applications: expression vectorshuttle vectorvector permitting construction of fusion proteins  /  Depositors: A Matus

Permits and Restrictions

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Designations pBact-myc
Depositors A Matus
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.6180000305175780
DESCRIPTION OF VECTOR:
Intact vector size: 5.618
Type of vector: plasmid
Cloning sites: EcoRI ClaI
Polylinker sites: HindIII NcoI EcoRI ClaI HindIII
Construction: pBact16, pUC18, c-myc
Host range: Escherichia coli; mammalian cells
Features (with orientation and position when available):
promoter: beta-actin, ->
restriction site: HindIII
other: Kozak consensus sequence
restriction site: NcoI
other: c-myc epitope sequence, ->
restriction site: EcoRI
restriction site: ClaI
restriction site: HindIII
terminator: SV40 polyadenylation
replicon: pMB1
marker(s): ampR, <-
Vector: pBact-myc (plasmid)
Promoters: Promoter beta-actin
Construction: pBact16, pUC18, c-myc
Marker(s):ampR
Construct size (kb): 5.618000030517578
Features: marker(s): ampR
other: Kozak consensus sequence
other: c-myc epitope sequence
promoter: beta-actin
replicon: pMB1
restriction site: ClaI
restriction site: EcoRI
restriction site: HindIII
restriction site: NcoI
terminator: SV40 polyadenylation
Applications
expression vector
shuttle vector
vector permitting construction of fusion proteins
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--5.6, PstI--4.8, 0.45, 0.35; EcoRI--5.6.
Shuttle expression vector allowing production of a fusion protein containing an amino terminal c-myc epitope tag (FQKLISEEDLN), that can be used for immunological detection of the recombinant protein in transfected cells.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Cravchik A, Matus A. A novel strategy for the immunological tagging of cDNA constructs. Gene 137: 139-143, 1993. PubMed: 7506688

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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