pBM2240 (ATCC® 87021)

Applications: shuttle vectorvector for other uses  /  Depositors: M Johnston

Permits and Restrictions

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Designations pBM2240
Depositors M Johnston
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.2500000000000000
Vector: pBM2240 (phagemid)
Construction: pRS316, lambdaMG3
Marker(s):URA3,ampR
Construct size (kb): 7.25
Features: marker(s): ampR, URA3
replicon: pMB1, CEN6/ARS4
centromere: CEN6
Applications
shuttle vector
vector for other uses
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--7.3; KpnI--5.6, 1.5; BamHI/XbaI--6.4, 0.9.
S. cerevisiae is cotransformed with the lambda clone of interest and vector linearized with an EcoRI/XhoI double digest. Ura+ colonies are tested for complementation of a mutant phenotype.
YC-type shuttle vector designed to clone yeast genes that are difficult to clone by rescuing inserts from the vector lambdaMG3 by homologous recombination.
Constructed by insertion of a 0.858 kb BamHI-XbaI fragment and a 1.5 kb KpnI fragment from lambdaMG3 into the corresponding sites of pRS316 (ATCC 77145).
The order of the major features in this plasmid is: f1 ori - lacZ - T7 promoter - KpnI/lambdaMG3/KpnI - MCS - BamHI/lambdaMG3/XbaI - T3 promoter - pMB1 ori - ampR - CEN6/ARS4 - URA3.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Erickson JR, Johnston M. Direct cloning of yeast genes from an ordered set of lambda clones in Saccharomyces cerevisiae by recombination in vivo. Genetics 134: 151-157, 1993. PubMed: 8514124

Shipped freeze-dried
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