pBM2240 (ATCC® 87021)

Applications: shuttle vectorvector for other uses  /  Depositors: M Johnston

Permits and Restrictions

View Permits

Designations pBM2240
Depositors M Johnston
Biosafety Level 1
Vector Information
Size (kb): 7.2500000000000000
Vector: pBM2240 (phagemid)
Construction: pRS316, lambdaMG3
Marker(s):URA3,ampR
Construct size (kb): 7.25
Features: marker(s): ampR, URA3
replicon: pMB1, CEN6/ARS4
centromere: CEN6
Applications
shuttle vector
vector for other uses
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--7.3; KpnI--5.6, 1.5; BamHI/XbaI--6.4, 0.9.
S. cerevisiae is cotransformed with the lambda clone of interest and vector linearized with an EcoRI/XhoI double digest. Ura+ colonies are tested for complementation of a mutant phenotype.
YC-type shuttle vector designed to clone yeast genes that are difficult to clone by rescuing inserts from the vector lambdaMG3 by homologous recombination.
Constructed by insertion of a 0.858 kb BamHI-XbaI fragment and a 1.5 kb KpnI fragment from lambdaMG3 into the corresponding sites of pRS316 (ATCC 77145).
The order of the major features in this plasmid is: f1 ori - lacZ - T7 promoter - KpnI/lambdaMG3/KpnI - MCS - BamHI/lambdaMG3/XbaI - T3 promoter - pMB1 ori - ampR - CEN6/ARS4 - URA3.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Erickson JR, Johnston M. Direct cloning of yeast genes from an ordered set of lambda clones in Saccharomyces cerevisiae by recombination in vivo. Genetics 134: 151-157, 1993. PubMed: 8514124

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet