pJIR750 [JIR2438] (ATCC® 87015)

Applications: expression vectorshuttle vectorvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: JI Rood

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Designations pJIR750 [JIR2438]
Depositors JI Rood
Biosafety Level 1
Vector Information
Size (kb): 6.5679998397827150
Vector: pJIR750 (plasmid)
Promoters: Promoter lac
Construction: pJIR418
Marker(s):cmlR
Construct size (kb): 6.567999839782715
Features: insert detection: lacZ'
marker(s): catP
other: rep
promoter: lac
replicon: pIP404
replicon: pMB1
MCS: EcoRI...HindIII
Applications
expression vector
shuttle vector
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BglI--4.4, 2.2; ClaI--6.6; EcoRV--6.6.
The vector contains the following restriction sites (approximate kb from the EcoRI site): BglI--0.20, 2.50; ClaI--4.99, 5.03; EcoRV--4.72; NdeI--0.27, 2.09; PvuI--0.17; PvuII--0.14, 6.39; SmaI--4.98.
The chloramphenicol resistance gene (catP), expressed in both C. perfringens and E. coli, was derived from C. perfringens and is inactivated by insertions at the EcoRV site.
The standard reverse sequencing primer is not useful in this vector due to the duplication of the 181 bp region between pUC18 sites PvuII and EcoRI.
Shuttle expression vector derived from pJIR418 (ATCC 77387) permitting expression of antibiotic resistance in both hosts.
Media ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
References

Bannnam TL, Rood JI. Clostridium perfringens-Escherichia coli shuttle vectors that carry single antibiotic resistance determinants. Plasmid 29: 233-235, 1993. PubMed: 8356117

Shipped frozen
Shipping Information

 Frozen glycerol stock of E. coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87015