Size (kb): 6.5679998397827150
Vector: pJIR750 (plasmid)
Promoters: Promoter lac
Construct size (kb): 6.567999839782715
Features: insert detection: lacZ'
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BglI--4.4, 2.2; ClaI--6.6; EcoRV--6.6.
The vector contains the following restriction sites (approximate kb from the EcoRI site): BglI--0.20, 2.50; ClaI--4.99, 5.03; EcoRV--4.72; NdeI--0.27, 2.09; PvuI--0.17; PvuII--0.14, 6.39; SmaI--4.98.
The chloramphenicol resistance gene (catP), expressed in both C. perfringens and E. coli, was derived from C. perfringens and is inactivated by insertions at the EcoRV site.
The standard reverse sequencing primer is not useful in this vector due to the duplication of the 181 bp region between pUC18 sites PvuII and EcoRI.
Shuttle expression vector derived from pJIR418 (ATCC 77387)
permitting expression of antibiotic resistance in both hosts.
Bannnam TL, Rood JI. Clostridium perfringens-Escherichia coli shuttle vectors that carry single antibiotic resistance determinants. Plasmid 29: 233-235, 1993. PubMed: 8356117
Frozen glycerol stock of E. coli containing the plasmid.