lambdaSK6 [clone 23] (ATCC® 86965)

Applications: vector containing primer sites useful for sequencingvector for constructing genomic librariesvector for other usesvector permitting positive selection for insertsvector permitting visual detection of recombinants  /  Depositors: ER Zabarovsky, G Winberg, G Klein

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Designations lambdaSK6 [clone 23]
Depositors ER Zabarovsky, G Winberg, G Klein
Biosafety Level 1
Vector Information
Size (kb): 43.2000007629394500
Vector: lambdaSK6 (phage, lambda - replacement)
Promoters: Promoter lac
Construction: lambda 2001
Marker(s):red+ gam+
Construct size (kb): 43.20000076293945
Features: insert detection: lacZ', Spi+
marker(s): red+ gam+
promoter: lac
replicon: lambda
Applications
vector containing primer sites useful for sequencing
vector for constructing genomic libraries
vector for other uses
vector permitting positive selection for inserts
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 13.5, 9.0; EcoRI--20.0, 13.5, 9.0; XhoI--20.0, 13.5, 9.0.
Vector permitting positive selection (Spi) of and visual detection (lacZ') of recombinants. Useful for generating jumping and linking libraries. Also permits construction using partial filling-in.
The polylinker sites attached to each arm are the same.
The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7.
The order of the major features in this plasmid is: arm - XbaI/MCS/AvrII XbaI - lacZO --> - stuffer - <--lacZO - XbaI AvrII/MCS/XbaI - arm.
References

Zabarovsky ER, et al. Lambda SK diphasmids: phage lambda vectors for genomic, jumping, linking and cDNA libraries. Gene 127: 1-14, 1993. PubMed: 8486275

Zabarovsky ER, Winberg GN. Improved methods and new vectors for creating libraries of cloned genes. Sov. Sci. Rev. 11: 1-65, 1992.

Shipped freeze-dried
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