pJQ200SK (ATCC® 77483)

Applications: integrating vectorvector permitting construction of fusion proteinsvector permitting positive selection for integrationvector permitting visual detection of recombinants  /  Depositors: MF Hynes

Permits and Restrictions

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Designations pJQ200SK
Depositors MF Hynes
Biosafety Level 1
Vector Information
Size (kb): 5.4000000953674320
Vector: pJQ200SK (plasmid)
Promoters: Promoter lac
Construction: P15A ori, sacB, RP4, gtmR, pBluescriptSK MCS
Marker(s):sacB,gtmR
Construct size (kb): 5.400000095367432
Features: insert detection: lac
marker(s): gtmR, sacB
promoter: lac
replicon: P15A, oriT
Applications
integrating vector
vector permitting construction of fusion proteins
vector permitting positive selection for integration
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; KpnI--3.6, 1.8; SstI--5.4.
Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts.
A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g. as a BamHI cassette).
When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector.
Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration.
The order of the major features of the plasmid is: gtmR - SstI/MCS/KpnI - P15A - traJ - oriT - sacB.
Growth Conditions
Temperature: 37.0°C
References

Quandt J, Hynes MF. Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Gene 127: 15-21, 1993. PubMed: 8486283

Shipped frozen
Shipping Information Frozen glycerol stock of E. coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 77483