pUK4134 (ATCC® 77480)

Applications: contains sequence origin of transfer replicationcontains sequence ribosomal protein S12integrating vectorshuttle vectorvector permitting positive selection for integration  /  Depositors: SC Straley

Permits and Restrictions

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Designations pUK4134
Depositors SC Straley
Biosafety Level 1
Vector Information
Size (kb): 7.0000000000000000
Vector: pUK4134 (plasmid)
Construction: pRTP1, pJM703.1
Marker(s):ampR,strS
Construct size (kb): 6.699999809265137
Features: marker(s): ampR
marker(s): strS
other: lambda cos site
replicon: R6K
replicon: oriT
Applications
contains sequence origin of transfer replication
contains sequence ribosomal protein S12
integrating vector
shuttle vector
vector permitting positive selection for integration
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.7, 0.5; EcoRI--6.4, 0.8; EcoRV--7.2; SalI--7.2.
RP6 is a suicide replicon in hosts not producing the pir protein.
RpsL (streptomycin sensitive) is dominant to streptomycin resistance, so loss of the plasmid can identified by streptomycin resistance and ampicillin sensitivity.
Suicide, gene replacement vector for Yersiniae and other gram-negative hosts that permits positive selection for loss of the plasmid after allelic exchange.
The vector also contains cos sites and oriT, and can be used for cosmid cloning or conjugative transfer.
The order of the major features in the plasmid is: EcoRV - R6K ori - oriT - SalI - cos - rpsL - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Skrzypek E, et al. New suicide vector for gene replacement in Yersiniae and other gram-negative bacteria. Plasmid 29: 160-163, 1993. PubMed: 8469722

Stibitz S, et al. The construction of a cloning vector designed for gene replacement in Bordetella pertussis. Gene 50: 133-140, 1986. PubMed: 2884169

Miller VL, Mekalanos JJ. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170: 2575-2583, 1988. PubMed: 2836362

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 77480