pI3 (ATCC® 77461)

Applications: shuttle vector  /  Depositors: KW Minton, C Masters

Permits and Restrictions

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Designations pI3
Depositors KW Minton, C Masters
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 16.0000000000000000
Vector: pI3 (plasmid)
Construction: pKK232-8, pUE10
Construct size (kb): 16.0
Features: marker(s): ampR, cmlR
replicon: pMB1, pUE10
shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--17.0; EcoRI--14.0, 4.3; HindIII--17.0.
Shuttle vector using ampR selection in E. coli and cmlR selection in Deinococcus radiodurans.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - pUE10 replicon - pUE10 promoter -> - cmlR -> - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Masters CI, Minton KW. Promoter probe and shuttle plasmids for Deinococcus radiodurans. Plasmid 28: 258-261, 1992. PubMed: 1461940

Shipped freeze-dried