pBJ-247 [pBS-GAL1/10] (ATCC® 77453)

Applications: contains easily purifiable cassette(s) for constructionshuttle vectorvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNA  /  Depositors: C Hug

Permits and Restrictions

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Designations pBJ-247 [pBS-GAL1/10]
Depositors C Hug
Biosafety Level 1
Vector Information
Size (kb): 3.7000000476837160
Vector: pBJ-247 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript II SK+
Marker(s):ampR
Construct size (kb): 3.700000047683716
Features: marker(s): ampR
promoter for expression: GAL10...GAL1
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: BamHI...SacI
MCS: KpnI...EcoRI
Applications
contains easily purifiable cassette(s) for construction
shuttle vector
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.7; EcoRI--3.7; SacI--3.7.
The GAL10 RNA start site is disrupted, thus providing only one direction of transcription. Unless a start site for transcription is provided, little or no message should be made from inserts cloned into the GAL10 side of the promoter cassette.
No pBS polylinker sites are included in the GAL1/GAL10 promoter cassette.
Plasmid containing a BamHI-GAL1/GAL10-EcoRI cassette of 685 bp useful for making other constructions.
The order of the major features in this plasmid is: f1 ori - lacZ' - T7 promoter - Kpn1/MCS/BamHI - GST-GAL1 promoter - GAL10 promoter - EcoRI/MCS/SacI - T3 promoter - lacI - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Christopher Hug, personal communication

Shipped freeze-dried
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