pRB381 (ATCC® 77377)

Applications: shuttle vectorvector permitting visual detection of activity in colonies  /  Depositors: R Bruckner

Permits and Restrictions

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Designations pRB381
Depositors R Bruckner
Biosafety Level 1
Vector Information
Size (kb): 8.8000001907348630
Vector: pRB381 (plasmid)
Construction: pUB110, pBR322, lacZ
Marker(s):ampR,bleR,neoR
Construct size (kb): 8.800000190734863
Features: insert detection: lac
marker(s): ampR, neoR, bleR
replicon: pUB110, pMB1
terminator: rrnB, to phage lambd
Applications
shuttle vector
vector permitting visual detection of activity in colonies
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/BglI--5.0, 2.2, 1.1, 0.8; BamHI--9.4; BglI/BglII--3.5, 2.2, 2.0, 1.3.
Structural stability of the plasmid in B. subtilis can be affected by high levels of protein production. Under these conditions, cell growth and stability may be improved by reducing the antibiotic concentration in the media.
May not be suitable for cloning very strong expression signals.
Translational fusion shuttle vector using 'lacZ as reporter. Also useful for the construction of fusion proteins.
Neo confers resistance to neomycin and kanamycin and ble confers resistance to bleomycin and phleomycin.
The 5' truncated 'lacZ gene, lacking transcriptional and translational initiation signals, is useful in both E. coli and in B. subtilis, since the endogenous beta-galactosidase level in B. subtilis is low.
The order of the major features in the plasmid is: To terminator - HindIII/MCS/BamHI - 'lacZ - rrnB terminator - ampR - pMB1 ori - pUB110 ori - neoR - bleR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Bruckner R. A series of shuttle vectors for Bacillus subtilis and Eschericia coli. Gene 122: 187-192, 1992. PubMed: 1452028

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet