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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 43.3400001525878900 Vector: lambdagt102 (phage, lambda - replacement) Construction: lambdagt10 Construct size (kb): 43.34000015258789 Features: insert detection: imm434 cI
replicon: lambda
repressor gene: imm434 cI
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Applications
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vector for constructing cDNA libraries vector permitting positive selection for inserts
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Comments
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Restriction digests of the clone give the following sizes (kb): HindIII-->23.0, 9.6, 6.6; BamHI--19.4, 12.0, 6.2, 6.0, 1.5; PstI--19.4, 6.0, 3.2, 2.7, 2.1, 1.8, 1.25, 1.2, 1.1, 0.95, 0.9, 0.8, 0.6, 0.5, 0.4, 0.3, 0.2, 0.2; EcoRI--33.5, 10.5. DNA inserted into the cloning sites interrupts the imm434 cI repressor, and thus plaque formation of nonrecombinants is repressed on high-frequency lysogeny mutant strains such as C600hflA or BNN102. Differs from lambdagt101 ( ATCC 77363) only in the cI434 region. May not be distributed for commercial use. Cloning vector permitting positive selection of inserts and useful for construction of jumping clones and libraries. Does not cross-hybridize to sequences found in common plasmid vectors.
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References
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Lee J, Weissman SM. Construction of lambda gt103, a derivative of phage lambda gt10 that has unique EcoRI, NotI, SacI and SpeI sites and retains positive selection for recombinants. Gene 120: 85-88, 1992. PubMed: 1398127
Jinseon Lee, personal communication
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