lambdagt101 (ATCC® 77363)

Applications: vector for constructing cDNA librariesvector permitting positive selection for inserts  /  Depositors: J Lee, SM Weissman

Permits and Restrictions

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Designations lambdagt101
Depositors J Lee, SM Weissman
Biosafety Level 1
Vector Information
Size (kb): 43.3400001525878900
Vector: lambdagt101 (phage, lambda - replacement)
Construction: lambdagt10
Construct size (kb): 43.34000015258789
Features: insert detection: imm434 cI
replicon: lambda
repressor gene: imm434 cI
Applications
vector for constructing cDNA libraries
vector permitting positive selection for inserts
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--33.4, 11.1; PstI--21.0, 5.7, 3.0, 2.6, 2.1, 1.75, 1.25, 1.2, 1.1, 0.95, 0.8, 0.6 +smaller; HindIII-->23.0, 9.6, 7.6; BamHI--17.1, 12.5, 7.8, 6.8, 1.5.
DNA inserted into the cloning sites interrupts the imm434 cI repressor, and thus plaque formation of nonrecombinants is repressed on high-frequency lysogeny mutant strains such as C600hflA or BNN102.
May not be distributed for commercial use.
Differs from lambdagt101 (ATCC 77363) only in the cI434 region.
The genotype appears to be more stable in the cI434 region than lambdagt10.
Cloning vector permitting positive selection of inserts and useful for construction of jumping clones and libraries. Does not cross-hybridize to sequences found in common plasmid vectors.
References

Lee J, Weissman SM. Construction of lambda gt103, a derivative of phage lambda gt10 that has unique EcoRI, NotI, SacI and SpeI sites and retains positive selection for recombinants. Gene 120: 85-88, 1992. PubMed: 1398127

Jinseon Lee, personal communication

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