pLR60 (ATCC® 77335)

Applications: contains sequence regulator, positive, of GAL1, GAL2, GAL7, GAL10, and MEL1expression vectorvector permitting construction of fusion proteins  /  Depositors: G Lozano

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Designations pLR60
Depositors G Lozano
Biosafety Level 1
Vector Information
Size (kb): 3.3499999046325680
Vector: pLR60 (plasmid)
Promoters: Promoter T3
Construction: pBluescript SK-, GAL4
Marker(s):ampR
Construct size (kb): 3.349999904632568
Features: marker(s): ampR
promoter: T3, T7
replicon: pMB1
Applications
contains sequence regulator, positive, of GAL1, GAL2, GAL7, GAL10, and MEL1
expression vector
vector permitting construction of fusion proteins
Comments
Restriction digests of the clone give the following sizes (kb): SalI--3.35; SacI/KpnI--2.8, 0.5; XhoI--3.1, 0.25.
Convenient cloning vector allowing generation of a fusion protein with the DNA-binding domain of GAL4 (aa 3-147).
Multiple cloning sites present at both ends of the GAL4 segment permit in-frame fusions with the DNA-binding domain in the middle or at the C-terminus of the fusion product.
pBR322 was modified by deletion of bp 75-2352, destruction (by filling in) of the HindIII site, deletion of a 100 bp AatII/ClaI fragment (nt 4285 and 22 of pBR322), and inserting an XbaI linker into the latter deletion junction.
Transcription from the T3 promoter gives the sense strand.
The order of the major features of this plasmid is: pMB1 ori - lacI (non-functional) - lacZ'/ - T3 promoter - SacI/MCS/SpeI - GAL4 - ClaI/MCS/KpnI - T7 promoter - /lacZ' - f1 ori - ampR.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Raycroft L, Lozano G. A convenient cloning vector containing the GAL4 DNA-binding domain. Gene 118: 143-144, 1992. PubMed: 1511878

Laughon A, Gesteland RF. Primary structure of the Saccharomyces cerevisiae GAL4 gene. Mol. Cell. Biol. 4: 260-267, 1984. PubMed: 6366516

Shipped freeze-dried
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