pC4ro3 (ATCC® 77319)

Applications: contains sequence roughexpression vectorin another host, produces protein roughreporter constructshuttle vector  /  Depositors: TJ Lockett

Permits and Restrictions

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Designations pC4ro3
Depositors TJ Lockett
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 10.8000001907348600
Vector: pC4ro3 (plasmid)
Construction: pro1, Carnegie 4, pSL1180 MCS
Construct size (kb): 10.80000019073486
Features: marker(s): ampR
replicon: pMB1
contains sequence rough
expression vector
in another host, produces protein rough
reporter construct
shuttle vector
Restriction digests of the clone give the following sizes (kb): SalI--8.6, 2.2; HindIII--7.7, 3.1; PstI--10.8.
The SphI/HindIII fragment corresponding to the rough gene contains the following restriction sites (kb from the 5' end): BamHI--3.02; BglII--5.39; EcoRI--0.91, 4.91; SacI--0.57, 4.19, 5.86; SalI--1.80, 3.91; SmaI--4.43.
Vector containing a non-autonomous P-element and incorporating the rough gene (ro) as a dominant marker for P-element mediated gene transfer into the germ line of Drosophila melanogaster.
The order of the major features in this plasmid is: HindIII - P-element/Carnegie 4/P-element - XhoI/MCS/SphI - ro gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Lockett TJ, et al. The rough (ro+) gene as a dominant P-element marker in germ line transformation of Drosophila melanogaster. Gene 114: 187-193, 1992. PubMed: 1601302

Trevor J Lockett, personal communication

Shipped freeze-dried
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