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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 5.5999999046325680 Vector: pTEX (plasmid) Promoters: Promoter T3 Construction: T. cruzi gGAPDH, pMC1neo, pBluescript KS Marker(s):G418R,ampR,neoR Construct size (kb): 5.599999904632568 Features: marker(s): ampR, neoR, G418R
promoter: gGAPDH I, T3, T7
replicon: pMB1
terminator: gGAPDH II
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Applications
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expression vector shuttle vector vector permitting RNA synthesis in vitro
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Comments
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Restriction digests of the clone give the following sizes (kb): PstI--4.8, 0.65, 0.2; BamHI--5.6; EcoRI--5.6; KpnI--5.6. The neoR gene can be used as a selectable marker in Trypanosoma cruzi and Leishmania. Increasing the concentration of G418 in the media increases copy number and expression. Shuttle expression vector for Trypanosoma cruzi and Leishmania. Transfected DNA is maintained in transformed parasites extrachromosomally as circular head-to-tail tandem repeats. DNA can be rescued from transformed parasites and propagated in E. coli after linearizing and religation to produce single circular copies. The order of the major features in this plasmid is: SacI - SacII - 5' flanking region of gGAPDH I - SpeI/MCS/XhoI - PstI - gGAPDH I/gGAPDH II intergenic region - neoR - gGAPDH II 3' flanking region - KpnI - ampR - pBluescript KS.
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References
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Kelly JM, et al. A shuttle vector which facilitates the expression of transfected genes in Trypanosoma cruzi and Leishmania. Nucleic Acids Res. 20: 3963-3969, 1992. PubMed: 1324472
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