pJAK17 [JK397] (ATCC® 77292)

Applications: expression vectorshuttle vector  /  Depositors: JA Kornacki

Permits and Restrictions

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Designations pJAK17 [JK397]
Depositors JA Kornacki
Biosafety Level 1
Vector Information
Size (kb): 12.6000003814697300
Vector: pJAK17 (plasmid)
Promoters: Promoter tac
Construction: pMMB67HE (ATCC 37623), pSa
Marker(s):cmlR
Construct size (kb): 12.60000038146973
Features: marker(s): cmlR
promoter: tac
replicon: RSF1010
replicon: oriT
replicon: oriV
repressor gene: lacIq
MCS: HindIII...EcoRI
transcription terminator: rrnB
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--12.6; HindIII--12.6; SphI--9.0, 3.6.
A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria.
Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids.
Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013 (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria.
Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation.
Differs from pJAK16 (ATCC 77291) only in the orientation of the polylinker.
Media Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
References

Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457

Jon A Kornacki, personal communication

Related Products
component of:ATCC 87055
Shipped frozen
Shipping Information Freeze dried plasmid in E. coli

Product Sheet
Product Sheet
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Attachments Vector map for 77292