pJAK17 [JK397] (ATCC® 77292)

Applications: expression vectorshuttle vector  /  Depositors: JA Kornacki

Permits and Restrictions

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Designations pJAK17 [JK397]
Depositors JA Kornacki
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 12.6000003814697300
Vector: pJAK17 (plasmid)
Promoters: Promoter tac
Construction: pMMB67HE (ATCC 37623), pSa
Marker(s):cmlR
Construct size (kb): 12.60000038146973
Features: marker(s): cmlR
promoter: tac
replicon: RSF1010
replicon: oriT
replicon: oriV
repressor gene: lacIq
MCS: HindIII...EcoRI
transcription terminator: rrnB
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--12.6; HindIII--12.6; SphI--9.0, 3.6.
A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria.
Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids.
Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013 (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria.
Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation.
Differs from pJAK16 (ATCC 77291) only in the orientation of the polylinker.
Media ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
References

Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457

Jon A Kornacki, personal communication

Related Products
component of:ATCC 87055
Shipped frozen
Shipping Information Freeze dried plasmid in E. coli

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 77292