pJAK14 [JK388] (ATCC® 77289)

Applications: expression vectorshuttle vector  /  Depositors: JA Kornacki

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Designations pJAK14 [JK388]
Depositors JA Kornacki
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli SCS1
Vector Information
Size (kb): 11.0000000000000000
DESCRIPTION OF VECTOR:
Intact vector size: 11.000
Type of vector: plasmid
Cloning sites: EcoRI SacI Ecl136II KpnI BamHI XbaI HindIII
Polylinker sites: EcoRI SacI Ecl136II KpnI XmaI SmaI BamHI XbaI AccI SalI
HincII PstI SphI HindIII
Construction: pMMB67EH (ATCC 37622), Tn5
Host range: broad host range; Escherichia coli
Features (with orientation and position when available):
promoter: tac
MCS: EcoRI...HindIII, ->
transcription terminator: rrnB
marker(s): kanR (neoR)
replicon: oriV
replicon: oriT
replicon: RSF1010
repressor gene: lacIq
Vector: pJAK14 (plasmid)
Promoters: Promoter tac
Construction: pMMB67EH (ATCC 37622), Tn5
Marker(s):kanR (neoR)
Construct size (kb): 11.0
Features: marker(s): kanR (neoR)
promoter: tac
replicon: RSF1010
replicon: oriT
replicon: oriV
repressor gene: lacIq
MCS: EcoRI...HindIII
transcription terminator: rrnB
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--11.0; HindIII--11.0; PstI--8.2, 1.85, 1.0.
A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria.
Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids.
Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013 (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria.
Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation.
Differs from pJAK15 (ATCC 77290) only in the orientation of the polylinker.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457

Jon A Kornacki, personal communication

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