pJAK13 [JK387] (ATCC® 77288)

Applications: expression vectorshuttle vector  /  Depositors: JA Kornacki

Designations pJAK13 [JK387]
Depositors JA Kornacki
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 10.8000001907348600
Vector: pJAK13 (plasmid)
Promoters: Promoter tac
Construction: pMMB67HE (ATCC 37623), R100.1
Marker(s):strR (spcR)
Construct size (kb): 10.80000019073486
Features: marker(s): strR (spcR)
promoter: tac
replicon: RSF1010
replicon: oriT
replicon: oriV
repressor gene: lacIq
MCS: HindIII...EcoRI
restriction site: SphI
transcription terminator: rrnB
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--10.8; HindIII--10.8; SphI--9.7, 1.1.
A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria.
Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids.
Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013 (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria.
Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation.
Differs from pJAK12 (ATCC 77287) only in the orientation of the polylinker.
Media ATCC® Medium 1445: Nutrient agar or broth with 20 mcg/ml streptomycin
Growth Conditions
Temperature: 37.0°C
References

Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457

Jon A Kornacki, personal communication

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