pNOT19 (ATCC® 77278)

Applications: vector permitting visual detection of recombinants  /  Depositors: HP Schweizer

Permits and Restrictions

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Designations pNOT19
Depositors HP Schweizer
Biosafety Level 1
Vector Information
Size (kb): 2.6940000057220460
Vector: pNOT19 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):ampR
Construct size (kb): 2.694000005722046
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
Applications
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; PvuI--1.75, 0.9; HindIII--2.7; NotI--2.7.
Available for research purposes only.
General purpose cloning vector containing a unique NotI site. Constructed for insertion of mobilization cassettes pMOB1-4 (ATCC 77280-77283) to produce a vector useful for allele replacement in Pseudomonas aeruginosa.
Also permits visual detection of recombinants by lacZalpha complementation. The alpha peptide is 107 aa.
Media Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Growth Conditions
Temperature: 37.0°C
References

Schweizer HP. Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker. Mol. Microbiol. 6: 1195-1204, 1992. PubMed: 1588818

Shipped freeze-dried
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