pNOT19 (ATCC® 77278)

Applications: vector permitting visual detection of recombinants  /  Depositors: HP Schweizer

Permits and Restrictions

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Designations pNOT19
Depositors HP Schweizer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.6940000057220460
Vector: pNOT19 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):ampR
Construct size (kb): 2.694000005722046
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
Applications
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; PvuI--1.75, 0.9; HindIII--2.7; NotI--2.7.
Available for research purposes only.
General purpose cloning vector containing a unique NotI site. Constructed for insertion of mobilization cassettes pMOB1-4 (ATCC 77280-77283) to produce a vector useful for allele replacement in Pseudomonas aeruginosa.
Also permits visual detection of recombinants by lacZalpha complementation. The alpha peptide is 107 aa.
Media ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Growth Conditions
Temperature: 37.0°C
References

Schweizer HP. Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker. Mol. Microbiol. 6: 1195-1204, 1992. PubMed: 1588818

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet