pOM2 (ATCC® 77221)

Applications: expression vectorvector containing primer sites useful for sequencingvector for constructing cDNA librariesvector permitting visual detection of recombinants  /  Depositors: I Oberbaumer

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Designations pOM2
Depositors I Oberbaumer
Biosafety Level 1
Vector Information
Size (kb): 2.7160000801086430
Vector: pOM2 (plasmid)
Promoters: Promoter lac
Construction: pUC8, M13tg131
Marker(s):ampR
Construct size (kb): 2.716000080108643
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
Applications
expression vector
vector containing primer sites useful for sequencing
vector for constructing cDNA libraries
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.7; HindIII--2.7; EcoRI--2.7; XhoI--2.7.
Escherichia coli transformed with this plasmid produce larger colonies when an insert is present in the polylinker than when there is no insert.
Expression vector useful for construction of cDNA libraries and containing priming sites useful for sequencing. Permits visual detection of recombinants by beta-galactosidase alpha peptide complementation.
pOM1 (ATCC 77220) and pOM2 (ATCC 77221) differ in the orientation of the polylinker.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975

Oberbaumer I. Blue libraries are beautiful. Focus 14: 72, 1992.

Ilse Oberbaumer, personal communication

Shipped freeze-dried
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