pFL61 (ATCC® 77215)

Applications: YE-type (episomal) shuttle vectorexpression vectorshuttle vector  /  Depositors: F Lacroute

Designations pFL61
Depositors F Lacroute
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.425
DESCRIPTION OF VECTOR:
Intact vector size: 5.425
Type of vector: plasmid
Cloning sites: NotI
Polylinker sites: EcoRI BanII SacI Asp718 KpnI AvaI SmaI XmaI BamHI XbaI
Construction: pUC19
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, URA3
replicon: pMB1, 2 micron
Vector: pFL61 (plasmid)
Construction: pUC19
Marker(s):URA3,ampR
Construct size (kb): 5.425
Features: marker(s): ampR, URA3
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; BglII--4.3, 1.1; ClaI--4.9, 0.5.
A YE-type shuttle expression vector.
The order of the major features in this plasmid is: EcoRI/MCS/XbaI - pGK5' - NotI - BstXI - BstXI - NotI - pGK3' - URA3 - 2 micron - pMB1 ori - AmpR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Minet M, et al. Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNA. Plant J. 2: 417-422, 1992. PubMed: 1303803

Francois Lacroute, personal communication

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Shipping Information Distributed: freeze-dried
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