pFL61 (ATCC® 77215)

Applications: YE-type (episomal) shuttle vectorexpression vectorshuttle vector  /  Depositors: F Lacroute

Permits and Restrictions

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Designations pFL61
Depositors F Lacroute
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.425
Intact vector size: 5.425
Type of vector: plasmid
Cloning sites: NotI
Polylinker sites: EcoRI BanII SacI Asp718 KpnI AvaI SmaI XmaI BamHI XbaI
Construction: pUC19
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, URA3
replicon: pMB1, 2 micron
Vector: pFL61 (plasmid)
Construction: pUC19
Construct size (kb): 5.425
Features: marker(s): ampR, URA3
replicon: pMB1, 2 micron
YE-type (episomal) shuttle vector
expression vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; BglII--4.3, 1.1; ClaI--4.9, 0.5.
A YE-type shuttle expression vector.
The order of the major features in this plasmid is: EcoRI/MCS/XbaI - pGK5' - NotI - BstXI - BstXI - NotI - pGK3' - URA3 - 2 micron - pMB1 ori - AmpR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Minet M, et al. Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNA. Plant J. 2: 417-422, 1992. PubMed: 1303803

Francois Lacroute, personal communication

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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