pFL59+ (ATCC® 77211)

Applications: YC-type (centromeric) shuttle vectorshuttle vectorvector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: F Lacroute

Designations pFL59+
Depositors F Lacroute
Biosafety Level 1
Vector Information
Size (kb): 4.8010001182556150
Vector: pFL59+ (phagemid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):TRP1,ampR
Construct size (kb): 4.801000118255615
Features: insert detection: lacZ'
marker(s): ampR, TRP1
promoter: lac
replicon: pMB1, f1, ARS-CEN6
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
shuttle vector
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.8; BglII--4.0, 0.85; ClaI--4.0, 0.8.
YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - TRP1 - ARS-CEN6 - pMB1 ori - ampR - f1 ori.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470

Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589

Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827

Dotto GP, et al. The functional origin of Bacteriophage f1 DNA replication. J. Mol. Biol. 172: 507-521, 1984. PubMed: 6607361

Panzeri L, Philippsen P. Centromeric DNA from chromosome VI in Saccharomyces cerevisiae. EMBO J. 1: 1605-1611, 1982. PubMed: 6327282

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map