pSVbeta plasmid in E. coli (ATCC® 77176)

Applications: contains easily purifiable cassette(s) for constructionreporter constructvector for other usesvector permitting visual detection of activity in colonies  /  Depositors: GR Macgregor

Permits and Restrictions

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Designations pSVbeta plasmid in E. coli
Depositors GR Macgregor
Biosafety Level 1
Vector Information
Size (kb): 7.00
Vector: pSVbeta (plasmid)
Promoters: Promoter SV40 early
Construction: pUC19, pC4AUG beta-gal, pL1
Marker(s):ampR
Construct size (kb): 7.0
Features: insert detection: lacZ'
marker(s): ampR
promoter: SV40 early
replicon: pMB1
terminator: SV40 late
enhancer: SV40 early
Applications
contains easily purifiable cassette(s) for construction
reporter construct
vector for other uses
vector permitting visual detection of activity in colonies
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.0; HindIII--7.0; EcoRI/HindIII--4.3, 2.7; NotI--3.6 (doublet); BamHI--3.6, 3.2, 0.2.
Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining or fluorometric assay.
Designed to express high levels of beta-galactosidase or an alternative gene product in COS cells. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors.
The lacZ gene may be excised by NotI and replaced with alternative sequences.
Recommended ampicillin concentration is 200 ug/ml.
The order of the major features in this plasmid is: pUC19 - EcoRI - SV40 enhancer - SV40 early promoter - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - HindIII - pUC19.
Media ATCC® Medium 1945: Terrific broth (ATCC medium 1743) with 200 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

MacGregor GR, Caskey CT. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17: 2365, 1989. PubMed: 2495524

Grant R Macgregor, personal communication

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