pCMVlacZII (ATCC® 77169)

Applications: reporter constructshuttle vector  /  Depositors: LA Culp

Permits and Restrictions

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Designations pCMVlacZII
Depositors LA Culp
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli DH5alpha
Vector Information
Size (kb): 10.8999996185302700
Intact vector size: 10.900
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): neoR
promoter: CMV, RSV
replicon: pMB1, M13, SV40, polyoma
terminator: SV40
Vector: pCMVlacZII (plasmid)
Promoters: Promoter CMV
Construct size (kb): 10.89999961853027
Features: marker(s): neoR
promoter: CMV, RSV
replicon: pMB1, M13, SV40, polyoma
terminator: SV40
reporter construct
shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 1.3; HindIII/XbaI--7.2, 3.8; KpnI--7.4, 3.6; StuI--11.0.
Shuttle reporter plasmid permitting visual detection of activity by histochemical staining.
Presence of beta-galactosidase activity is used to follow cell lineage in culture or in situ.
The order of the major features in this plasmid is: CMV promoter - bacterial lacZ - polyadenylation signal - RSV promoter - neoR - polyoma virus ori - SV40 ori - pMB1 ori - M13 ori.
Media ATCC® Medium 1799: LB medium (ATCC medium 1065) plus 10 mcg/ml neomycin
Growth Conditions
Temperature: 37.0°C

Jo H, et al. Differential effect of shear stress on extracellular signal-regulated kinase and N-terminal Jun kinase in endothelial cells. Gi2- and Gbeta/gamma-dependent signaling pathways. J. Biol. Chem. 272: 1395-1401, 1997. PubMed: 8995450

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