pCMVlacZII (ATCC® 77169)

Applications: reporter constructshuttle vector  /  Depositors: LA Culp

Permits and Restrictions

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Designations pCMVlacZII
Depositors LA Culp
Biosafety Level 1
Host
Distribution host: Escherichia coli DH5alpha
Vector Information
Size (kb): 10.8999996185302700
DESCRIPTION OF VECTOR:
Intact vector size: 10.900
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): neoR
promoter: CMV, RSV
replicon: pMB1, M13, SV40, polyoma
terminator: SV40
Vector: pCMVlacZII (plasmid)
Promoters: Promoter CMV
Marker(s):neoR
Construct size (kb): 10.89999961853027
Features: marker(s): neoR
promoter: CMV, RSV
replicon: pMB1, M13, SV40, polyoma
terminator: SV40
Applications
reporter construct
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 1.3; HindIII/XbaI--7.2, 3.8; KpnI--7.4, 3.6; StuI--11.0.
Shuttle reporter plasmid permitting visual detection of activity by histochemical staining.
Presence of beta-galactosidase activity is used to follow cell lineage in culture or in situ.
The order of the major features in this plasmid is: CMV promoter - bacterial lacZ - polyadenylation signal - RSV promoter - neoR - polyoma virus ori - SV40 ori - pMB1 ori - M13 ori.
Media ATCC® Medium 1799: LB medium (ATCC medium 1065) plus 10 mcg/ml neomycin
Growth Conditions
Temperature: 37.0°C
References

Jo H, et al. Differential effect of shear stress on extracellular signal-regulated kinase and N-terminal Jun kinase in endothelial cells. Gi2- and Gbeta/gamma-dependent signaling pathways. J. Biol. Chem. 272: 1395-1401, 1997. PubMed: 8995450

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Shipping Information Distributed: freeze-dried
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