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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 8.30 Vector: pRS317 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pRSS56 [pBluescript KS+, pBS(+)] Marker(s):ampR,LYS2 Construct size (kb): 8.30 Features: insert detection: lacZ'
marker(s): LYS2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
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Applications
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YC-type (centromeric) shuttle vector plasmid shuffling vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants
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Comments
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Restriction digests of the clone give the following sizes (kb): XbaI--8.4; PstI--8.4; EcoRI--8.4; HindIII--8.4. There are restriction sites for KpnI (2 sites), SpeI (2 sites), and EcoRV (1 site) in the Lys2 gene that are also present in the polylinker. One of a series of pBluescript-based centromere vectors (ATCC 77142-77145, 77157-77158) differing in the yeast selectable marker gene. YC-type centromere vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. Useful in plasmid shuffle experiments. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). The order of the major features in this plasmid is: LYS2 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori bla - CEN6 - ARSH4.
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References
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Sikorski RS, Boeke JD. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant gene. Methods Enzymol. 194: 302-318, 1991. PubMed: 2005795
Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436
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