YCF4 (ATCC® 77156)

Applications: shuttle vector  /  Depositors: P Hieter

Permits and Restrictions

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Designations YCF4
Depositors P Hieter
Biosafety Level 1
Vector Information
Size (kb): 8.5000000000000000
Vector: YCF4 (plasmid)
Construction: YCF3
Marker(s):URA3,ampR,SUP11
Construct size (kb): 8.5
Features: marker(s): ampR, URA3, SUP11
replicon: pMB1
centromere: CEN4
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--8.5; HindIII--8.5; BamHI--8.5; SalI (uncut).
The sequence to be mapped is cloned into the MCS of both YCF3 (ATCC 77155) and YCF4 (ATCC 77156) (both orientations being produced). NotI is used to linearize the constructs, which are transformed independently into YPH49.
YCF4 is used to recover sequences distal to the mapping sequence.
Shuttle chromosome fragmentation vector.
Constructed by inserting a 1.4 kb fragment containing CEN4 at the SalI site (blunt-ended) of YCF3.
The order of the major features in this plasmid is: Y' - MCS - CEN4 - URA3 - SUP11 - rep - pMB1 ori - bla.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Vollrath D, et al. Physical mapping of large DNA by chromosome fragmentation. Proc. Natl. Acad. Sci. USA 85: 6027-6031, 1988. PubMed: 3045811

Gerring SL, et al. Positional mapping of genes by chromosome blotting and chromosome fragmentation. Methods Enzymol. 194: 57-77, 1991. PubMed: 2005810

Shipped freeze-dried
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