pAB366Ben (ATCC® 77136)

Applications: contains sequence tubulin, betain another host, produces protein tubulin, betareporter constructshuttle vector  /  Depositors: BM Tyler

Permits and Restrictions

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Designations pAB366Ben
Depositors BM Tyler
Biosafety Level 1
Vector Information
Size (kb): 7.1999998092651370
Vector: pAB366Ben (plasmid)
Construction: pAB366, pBT6
Marker(s):ampR,bleR,benR
Construct size (kb): 7.199999809265137
Features: marker(s): ampR, bleR, benR
replicon: pMB1
Applications
contains sequence tubulin, beta
in another host, produces protein tubulin, beta
reporter construct
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 2.1; HindIII--4.0, 3.2; BamHI/HindIII--2.6, 2.5, 1.5, 0.65; BamHI/XhoI--3.0, 1.35, 1.2, 0.9, 0.8; SalI/HindIII--3.3, 2.6, 0.85, 0.55.
A 2.5 kb SalI/HindIII fragment contains the complete tubulin gene and 5' and 3' flanking regions.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Austin B, et al. Optimized vectors and selection for transformation of Neurospora crassa and Aspergillus nidulans to bleomycin and phleomycin resistance. Gene 93: 157-162, 1990. PubMed: 1699844

Orbach MJ, et al. Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol. Cell. Biol. 6: 2452-2461, 1986. PubMed: 2946938

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

Shipped frozen
Shipping Information  Frozen glycerol stock of Escherichia coli containing the plasmid.
Product Sheet
Product Sheet
Product Sheet