pAB366Ben (ATCC® 77136)

Applications: contains sequence tubulin, betain another host, produces protein tubulin, betareporter constructshuttle vector  /  Depositors: BM Tyler

Designations pAB366Ben
Depositors BM Tyler
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.1999998092651370
Vector: pAB366Ben (plasmid)
Construction: pAB366, pBT6
Construct size (kb): 7.199999809265137
Features: marker(s): ampR, bleR, benR
replicon: pMB1
contains sequence tubulin, beta
in another host, produces protein tubulin, beta
reporter construct
shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 2.1; HindIII--4.0, 3.2; BamHI/HindIII--2.6, 2.5, 1.5, 0.65; BamHI/XhoI--3.0, 1.35, 1.2, 0.9, 0.8; SalI/HindIII--3.3, 2.6, 0.85, 0.55.
A 2.5 kb SalI/HindIII fragment contains the complete tubulin gene and 5' and 3' flanking regions.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Austin B, et al. Optimized vectors and selection for transformation of Neurospora crassa and Aspergillus nidulans to bleomycin and phleomycin resistance. Gene 93: 157-162, 1990. PubMed: 1699844

Orbach MJ, et al. Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol. Cell. Biol. 6: 2452-2461, 1986. PubMed: 2946938

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

Shipped frozen
Shipping Information  Frozen glycerol stock of Escherichia coli containing the plasmid.