pUFR034 (ATCC® 77122)

Applications: shuttle vectorvector for constructing genomic librariesvector permitting visual detection of recombinants  /  Depositors: DW Gabriel

Permits and Restrictions

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Designations pUFR034
Depositors DW Gabriel
Biosafety Level 1
Vector Information
Size (kb): 8.6999998092651370
Vector: pUFR034 (cosmid)
Promoters: Promoter lac
Construction: pUC18, pSa747, pHC79, pUCD550, pUC4Kan, pLAFR3
Marker(s):neoR
Construct size (kb): 8.699999809265137
Features: insert detection: lacZ'
marker(s): neoR
promoter: lac
replicon: oriW
Applications
shuttle vector
vector for constructing genomic libraries
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--8.7; PstI--6.4, 1.35, 1.2; SmaI--7.0, 1.8.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Uses interruption of lacZalpha.
Shuttle vector permitting visual detection of recombinants.
The partition locus from Agrobacterium plasmid pTAR stabilizes the plasmid in X. citri and X. campestris pvs. campestris, vesicatoria, malvacearum, and translucens grown in broth culture.
The order of the major features in this plasmid is: lacZalpha with MCS (pUC18) - oriW (pSa747, IncW) - cos (pHC79) - parA+ (pUCD550) - aphA (pUC4Kan) - mob(P) (pLAFR3, Mob+).
Media ATCC® Medium 1799: LB medium (ATCC medium 1065) plus 10 mcg/ml neomycin
Growth Conditions
Temperature: 30.0°C
References

DeFeyter R, et al. Small, stable shuttle vectors for use in Xanthomonas. Gene 88: 65-72, 1990. PubMed: 2341039

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet