pSZ62 (ATCC® 77120)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: JW Szostak

Permits and Restrictions

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Designations pSZ62
Depositors JW Szostak
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.0999999046325680
Vector: pSZ62 (plasmid)
Promoters: Promoter none
Construction: pBR322
Construct size (kb): 6.099999904632568
Features: marker(s): ampR, HIS3
promoter: none
replicon: pMB1
terminator: none
enhancer: none
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI--6.1; BamHI--4.4, 1.7; BglII--5.4, 0.7; SalI/XhoI--4.6, 1.5; EcoRI/SalI--3.7, 2.4; SalI--6.1.
Integration YI-type shuttle vector.
Constructed by inserting a 1.7 kb HIS3-containing BamHI fragment into the BamHI site of pBR322.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Orr-Weaver TL, et al. Yeast transformation: a model system for the study of recombination. Proc. Natl. Acad. Sci. USA 78: 6354-6358, 1981. PubMed: 6273866

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