pSZ62 (ATCC® 77120)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: JW Szostak

Permits and Restrictions

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Designations pSZ62
Depositors JW Szostak
Biosafety Level 1
Vector Information
Size (kb): 6.0999999046325680
Vector: pSZ62 (plasmid)
Promoters: Promoter none
Construction: pBR322
Marker(s):ampR,HIS3
Construct size (kb): 6.099999904632568
Features: marker(s): ampR, HIS3
promoter: none
replicon: pMB1
terminator: none
enhancer: none
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.1; BamHI--4.4, 1.7; BglII--5.4, 0.7; SalI/XhoI--4.6, 1.5; EcoRI/SalI--3.7, 2.4; SalI--6.1.
Integration YI-type shuttle vector.
Constructed by inserting a 1.7 kb HIS3-containing BamHI fragment into the BamHI site of pBR322.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Orr-Weaver TL, et al. Yeast transformation: a model system for the study of recombination. Proc. Natl. Acad. Sci. USA 78: 6354-6358, 1981. PubMed: 6273866

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