YEp437 [pRB698] (ATCC® 77119)

Applications: YE-type (episomal) shuttle vectorshuttle vector  /  Depositors: D Botstein

Permits and Restrictions

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Designations YEp437 [pRB698]
Depositors D Botstein
Biosafety Level 1
Vector Information
Size (kb): 13.9000005722045900
Vector: YEp437 (plasmid)
Construction: pSI4, YEp6
Marker(s):ampR,HIS3,LEU2-d
Construct size (kb): 13.90000057220459
Features: marker(s): ampR, LEU2-d, HIS3
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--12.0, 1.8; EcoRI--6.2, 4.4, 2.4, 0.8; SalI--14.0.
LEU2-d is a promoter-defective allele of the yeast LEU2 gene, which increases plasmid copy number to 200-300 copies in the presence of LEU2 selection (host grown in medium lacking leucine).
YE-type shuttle vector.
The order of the major features in this plasmid is: LEU2-d - ori(2 micron) - HIS3 - SalI site - ampR.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Ma H, et al. Plasmid construction by homologous recombination in yeast. Gene 58: 201-216, 1987. PubMed: 2828185

Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet