YEp437 [pRB698] (ATCC® 77119)

Applications: YE-type (episomal) shuttle vectorshuttle vector  /  Depositors: D Botstein

Permits and Restrictions

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Designations YEp437 [pRB698]
Depositors D Botstein
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 13.9000005722045900
Vector: YEp437 (plasmid)
Construction: pSI4, YEp6
Marker(s):ampR,HIS3,LEU2-d
Construct size (kb): 13.90000057220459
Features: marker(s): ampR, LEU2-d, HIS3
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--12.0, 1.8; EcoRI--6.2, 4.4, 2.4, 0.8; SalI--14.0.
LEU2-d is a promoter-defective allele of the yeast LEU2 gene, which increases plasmid copy number to 200-300 copies in the presence of LEU2 selection (host grown in medium lacking leucine).
YE-type shuttle vector.
The order of the major features in this plasmid is: LEU2-d - ori(2 micron) - HIS3 - SalI site - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Ma H, et al. Plasmid construction by homologous recombination in yeast. Gene 58: 201-216, 1987. PubMed: 2828185

Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781

Shipped freeze-dried