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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 6.1999998092651370 Vector: pBP103 (plasmid) Promoters: Promoter lac Construction: YTCA-1x, pRS303 ( ATCC 77138)Marker(s):ampR,HIS3 Construct size (kb): 6.199999809265137 Features: marker(s): ampR, HIS3
promoter: lac, SP6
replicon: pMB1
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Applications
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YAC fragmentation vector vector permitting RNA synthesis in vitro
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--4.6; EcoRI--4.6; SalI--4.6; SalI/EcoRI--4.4, 0.3. Generic chromosome fragmentation vector containing promoters for in vitro RNA synthesis. The unique cloning sites can be used to insert targeting sequences, such as cDNAs. Any unique restriction site between the targeting sequence and the telomere-adjacent sequence can be used to linearize the plasmid before transformation. The EcoRV and ClaI sites between the SP6 promoter and the telomere sequence can be used for recovery from deletion derivatives of YAC insert sequences adjacent to the fragmentation site. A 0.24 kb EcoRI/SalI fragment containing the telomere-adjacent sequence from YTCA-1x was ligated into the MCS of pSP73. The EcoRI and BamHI sites of the MCS were destroyed and a 0.3 kb NdeI/PvuII fragment was inserted into the PvuII site of pRS303. The order of the major features in this plasmid is: bla - HIS3 - lacZ'/MCS - HindIII - SphI - PstI - AccI - SalI - TEL - ClaI - EcoRV - SP6 promoter.
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References
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Pavan WJ, et al. High-efficiency yeast artificial chromosome fragmentation vectors. Gene 106: 125-127, 1991. PubMed: 1937033
Reeves RH, et al. Yeast artificial chromosome modification and manipulation. Methods Enzymol. 216: 584-603, 1992. PubMed: 1336105
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