pBP96 (ATCC® 77084)

Applications: YI-type (integrating) shuttle vector  /  Depositors: RH Reeves

Permits and Restrictions

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Designations pBP96
Depositors RH Reeves
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.5999999046325680
Vector: pBP96 (plasmid)
Construction: BLUR8, HIS3
Construct size (kb): 4.599999904632568
Features: marker(s): ampR, HIS3
replicon: pMB1
YI-type (integrating) shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 1.6; EcoRI--4.6; PstI--3.3, 1.3.
Digestion with BamHI removes the plasmid sequences from the Alu ends for targeting integration.
Transformation into pools from a YAC library derived from a human-mouse somatic cell hybrid, and selection for His+, resulted in an approximate 50-fold enrichment for Alu-containing YACs.
Larger YACs were preferentially selected. Some YACs demonstrated loss of Alu-containing restriction fragments suggesting internal deletions resulting when the vector targeted two Alu sequences in the YAC.
YI-type shuttle integrative vector targeting human Alu sequences in YAC libraries derived from somatic cell hybrids.
Constructed by inserting a blunt-ended 1.2 kb fragment containing S. cerevisiae HIS3 into the blunted MscI within the Alu sequence of BLUR8.
The order of the major features in this plasmid is: bla - BamHI - Alu - HIS3 - Alu - BamHI.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Pavan WJ, Reeves RH. Integrative selection of human chromosome-specific yeast artificial chromosomes. Proc. Natl. Acad. Sci. USA 88: 7788-7791, 1991. PubMed: 1881915

Reeves RH, et al. Yeast artificial chromosome modification and manipulation. Methods Enzymol. 216: 584-603, 1992. PubMed: 1336105

Roger H Reeves, personal communication

Shipped freeze-dried