pHCMV-G (ATCC® 75497)

Applications: cloning vector  /  Depositors: Regents of the University of California, J Yee, JC Burns, Regents of the University of California

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Designations pHCMV-G
Depositors Regents of the University of California, J Yee, JC Burns, Regents of the University of California
Biosafety Level 1
Vector Information
Size (kb): 6.3499999046325680
DESCRIPTION OF VECTOR:
Intact vector size: 6.350
Type of vector: plasmid
Cloning sites: XbaI, SalI, BamHI, XhoI, KpnI
Polylinker sites:
Construction: pBR322, HCMV, VSV-G
Host range: Homo sapiens; hamster; zebrafish; Escherichia coli
Features (with orientation and position when available):
promoter: HCMV (cytomegalovirus), ->
marker(s): ampR, ->
Vector: pHCMV-G (plasmid)
Promoters: Promoter HCMV (cytomegalovirus)
Construction: pBR322, HCMV, VSV-G
Marker(s):ampR
Construct size (kb): 6.349999904632568
Features: marker(s): ampR
promoter: HCMV (cytomegalovirus)
Applications
cloning vector
Comments
The retrovirus-derived vectors in which the retroviral envelope glycoprotein has been replaced by the G glycoprotein of vesicular stomatitis virus. Vector can be used in the transfer of an exogenous gene into the cells of non-mammalian organisms.
Contains the immediate early promoter of human cytomegalovirus, the cDNA encoding VSC-G protein and the polyademylation signal of the rabbit B-globin gene.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Burns JC. Generation, concentration and efficient transfer of VSV-G pseudotyped retroviral vectors. US Patent 5,512,421 dated Apr 30 1996

Shipped frozen
Shipping Information Distributed: DNA supplied by depositor (500 ng dried)
Product Sheet
Product Sheet
Product Sheet