pRG 173 (NT-4) (ATCC® 75131)

Depositors: Regeneron Pharmaceuticals, Inc., J Fandl, N Panayotatos, Regeneron Pharmaceuticals, Inc.

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Designations pRG 173 (NT-4)
Depositors Regeneron Pharmaceuticals, Inc., J Fandl, N Panayotatos, Regeneron Pharmaceuticals, Inc.
Biosafety Level 1
Vector Information
Marker(s):AmpR
Comments
Transformation of this plasmid into E. coli RFJ26 (which is lacIq) and induction of large scale cultures resulted in the expression of a biologically active form of recombinant human NT-4.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Panayotatos N. Modified LAM belta signal sequence and processes for producing recombinant neurotrophines. US Patent 5,389,529 dated Feb 14 1995

Product Sheet
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