pBIIHI.2KI [NEB560] (ATCC® 68002)

Applications: cloning vector  /  Depositors: New England Biolabs, Inc., PA Rees, New England Biolabs, Inc.

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Designations pBIIHI.2KI [NEB560]
Depositors New England Biolabs, Inc., PA Rees, New England Biolabs, Inc.
Biosafety Level 1
Vector Information
Size (kb): 5.5000000000000000
Vector: pBIIHI.2KI (plasmid)
Marker(s):ampR,strS
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.8, 1.55; EcoRI--5.6; HindIII--5.6; HpaI--3.6, 1.9.
The vector is derived from pNO1523 (ATCC 37095) by the insertion of a BglII linker at the EcoRI site, a HpaI linker at the PvuII site, and a KpnI linker at the EcoRV site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Benner JS, Rees P. Method for producing the HPA I restriction endonuclease and methylase. US Patent 5,298,404 dated Mar 29 1994

Phyllis A Rees, personal communication

Shipped freeze-dried
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